The construction of Resorts World Sentosa and Universal Studios Singapore began on 19 April 2008. After almost two years of construction, the park opened on 18 March 2010.

Universal Studios Singapore was opened for "sneak peek week" in view of the Chinese New Year Celebrations, from 5 pm to 9 pm every night between 14 and 21 February 2010. Though visitors had to pay SGD$10 to enter the park even-though rides were not operating, tickets for the week were sold out in 2 days. On 5 March 2010, it was announced that the park will open its doors at 8:28 am (UTC+8) on 18 March 2010, for a soft-opening phase. From 13 March 2010, the team members of Resorts World Sentosa and their families had a chance to visit the park before the public has a chance to do so. The park had its soft opening period from 18 March 2010 to 26 October 2010.

The official grand opening of the park was held on 28 May 2011, along with the "grand opening gala" held on 27 May 2011 evening. It had Asian personalities Jet Li, Maggie Cheung, Zhao Wei, and former American Idol judge Paula Abdul making an appearance at the gala event, along with some 1,600 guests.

On 21 October 2011, Universal Studios Singapore began their Halloween Horror Nights events.

On 3 April 2019, Genting Group announced the expansion of Universal Studios Singapore with two new themed areas, Minion Land and Super Nintendo World.